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. Author manuscript; available in PMC: 2016 Oct 5.
Published in final edited form as: Curr Biol. 2015 Sep 24;25(19):2591–2596. doi: 10.1016/j.cub.2015.08.039

Figure 3. Cep135mini expression displaces SAS-6, CPAP, and γ-tubulin from centrioles and centrosomes.

Figure 3

(A) Exogenous Cep135full and Cep135mini expression in S-phase arrested RPE1 cells decreases SAS-6 localization to centrioles. Exogenous Cep135full and Cep135mini expression causes a 48% and 44% decrease in SAS-6 levels, respectively. Upper panels depict SAS-6 (green) in non-transfected cells and lower panels show SAS-6 in Cep135mini transfected cells. Centrin (red) levels were not affected by expression of either protein. (B) Cep135mini expression in S-phase arrested RPE1 cells decreases CPAP localization to centrioles. Cep135mini expression causes a 90% decrease in CPAP levels. Cep135full expression causes an intermediate 43% decrease in CPAP levels. Upper panels depict CPAP (green) in non-transfected cells and lower panels show CPAP in Cep135mini transfected cells. (C) Exogenous Cep135mini, but not Cep135full, expression in cycling RPE1 cells causes a 52% decrease in γ-tubulin levels. Upper panels depict γ-tubulin (green) in non-transfected cells and lower panels show γ-tubulin in Cep135mini transfected cells. (A–C) Mean±SEM represents at least three separate experiments. Scale bar, 1 μm.