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. 2015 Oct 7;10(10):e0139462. doi: 10.1371/journal.pone.0139462

Fig 1. HED-fed, inbred obesity prone (OP-CD) rats have reduced responses to MTII.

Fig 1

(A) Weekly mean body weights of OP-CD strain Sprague-Dawley rats fed either HED or chow. When returned to chow, HED-fed rats defended their diet-induced obesity (DIO-D) relative to HED-naïve (HED-N) littermates. (B) Representative IPSC recordings from neurosecretory (NS) mpPVN cells from HED-N (left) and DIO-D (right) rats superimposed in control (solid gray), 100 nM MTII (black) and after washout (dashed gray). IPSCs are abolished by application of 50 μM bicuculline (dashed dark grey, left). Arrows to the left of traces indicate the relative direction and magnitude of MTII-mediated changes. MTII responses were significantly attenuated in PVN NS cells of DIO-D rats relative to HED-N rats. (C) Representative IPSC recordings from HED-N (left) and DIO-D (right) rats superimposed in control (solid gray), 300 nM NPY (black) and wash (dashed gray). Arrows to the left of the traces indicate the relative direction and magnitude of NPY-mediated IPSC changes. There was no significant difference between the effects of NPY in PVN NS cells of DIO-D and HED-N rats. ** p < 0.01.