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. 2015 Oct 7;10(10):e0139462. doi: 10.1371/journal.pone.0139462

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© 2015 Luchtman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) Representative darkfield photomicrographs showing in situ hybridization of MC4R mRNA gene expression in the DMN (outlined region) from DR (left) and DIO-D (right) outbred rats. Scale: 100 μm. 3V, third ventricle. (B) In situ hybridization for MC4R resulted in fewer silver grains over individual neurons in DMN, but not PVN or ARC of DIO-D vs. DR rats. Data were obtained from 9 DR and 5 DIO rats. Numbers of cells studied per animal: PVN—n = 113 ± 15 (DR), n = 97 ± 15 (DIO), p = 0.5; ARC—n = 61 ± 4 (DR), n = 60 ± 7 (DIO), p = 0.8; DMH—n = 127 ± 13 (DR), 92 ± 14 (DIO), p = 0.12. ** p < 0.01.