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. 2015 Oct 7;10(10):e0140266. doi: 10.1371/journal.pone.0140266

Fig 6. Gel pictures showing results of normal and semi quantitative reverse transcriptase PCR.

Fig 6

PCR amplified products of rol B (a), rol C (b) and nptII (c) gene are shown in the figure. Semi quantitative RT-PCR showing the relative expression of rol B (d) and rol C gene (e). β-actin used as housekeeping gene (f). TB1-TB4 represents rol b transgenics whereas TC1-TC3 represent rol C transgenics. WT stands for wild type plant of A. carvifolia, lane “P” refers to the plasmid DNA and lane M corresponds to the marker DNA (Fermentas). NC = negative control.