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. 2015 Oct 7;10(10):e0140064. doi: 10.1371/journal.pone.0140064

Fig 1. LysM and ERK1/2 mice express the expected mutations in germline DNA, but macrophages from ERK1/2 mice selectively retain expression of ERK2 protein.

Fig 1

(A) DNA from clipped tails of LysM and ERK1/2 mice (three of each) was amplified to detect the mutant or wild-type alleles for Lyz2, Erk1, and Erk2. (B) Macrophages were derived from bone marrow cultures stimulated for 7 d with LADMAC-conditioned medium containing M-CSF, and expression of ERK1 and ERK2 was detected by Western blotting. Diagonal arrows indicate the position of the ERK2 band, expressed in ERK1/2 macrophages but greatly reduced in Erk2 flox/flox Lyz2 Cre/Cre macrophages. Results are representative of three independent experiments. Labels: B6, C57BL/6J; E1 E2f/f, Erk1 -/- Erk2 flox/flox; E1, Erk1 -/-; E2, Erk2 flox/flox Lyz2 Cre/Cre.