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. 2015 Oct 7;10(10):e0140064. doi: 10.1371/journal.pone.0140064

Fig 7. Production of monocytes and macrophages by in vitro culture of Lin- progenitors from ERK1/2 and LysM bone marrow.

Fig 7

(A) Western blot of Lin- cells from LysM and ERK1/2 bone marrow. (B) Cytospin preparation showing representative cells generated by culture of Lin- cells for 4 d in 5 ng/ml IL–3, 10 ng/ml IL–6, and 10 ng/ml SCF (similar results were observed with M-CSF in addition to these growth factors). (C) Lin- cells were grown in IL–3, IL–6, and SCF and monitored daily for acquisition of neutrophil (CD11b+ Gr–1+) and monocyte (CD11b+ Gr–1-) lineage markers by flow cytometry. (D) Cells were grown and analyzed as in (C) but with the addition of 5 ng/ml M-CSF. (E) and (F) Total yields of cells from cultures of Lin- cells in myeloid-stimulating cytokines. Data in (A) and (B) are representative of two independent experiments; data in (C)-(F) show the means ± standard deviations of triplicate samples and are representative of two independent experiments.