Table 1. Antibodies used for flow cytometry.
| Antigen (alternate name) | Clone Name (isotype) | Fluorochrome conjugate | Vendor | Experimental Panel a |
|---|---|---|---|---|
| B220 (CD45R) | RA3-6B2 (rat IgG2a, κ) | PE-Cy7 | Biolegend | Lineage, FACS |
| CD3ε | 145-2C11 (Armenian hamster IgG1, κ) | PE-CF594 | BD Biosciences | Lineage |
| CD4 | RM4-5 (rat IgG2a, κ) | FITC | Biolegend | Lineage |
| CD8α | 53–6.7 (rat IgG2a, κ) | APC-H7 | BD Biosciences | Lineage |
| CD11b | M1/70 (rat IgG2b, κ) | BV421, Alexa Fluor 647, FITC, PE-Cy7 | Biolegend | Lineage, BMM, FACS, Differentiation |
| CD16/32 | 93 (rat IgG2a, λ) | APC-Cy7 | Biolegend | Progenitors |
| CD18 | M18/2 (rat IgG2a, κ) | PE | Biolegend | BMM |
| CD34 | RAM34 (rat IgG2a, κ) | eFluor 660 | eBioscience | Progenitors |
| CD115 (M-CSFR) | AFS98 (rat IgG2a, κ) | APC | Biolegend | BMM |
| CD117 (c-Kit) | 2B8 (rat IgG2b, κ) | Pacific Blue | Biolegend | Progenitors |
| CD127 (IL-7Rα) | A7R34 (rat IgG2a, κ) | PE-Cy7 | Biolegend | Progenitors |
| CD138 | 281–2 (rat IgG2a, κ) | BV421 | Biolegend | Lineage |
| F4/80 | BM8 (rat IgG2a, κ) | PE-Cy7 | Biolegend | BMM |
| Gr–1 (Ly-6G/Ly-6C) | RB6-8C5 (rat IgG2b, κ) | PerCP-Cy5.5, APC | Biolegend | Lineage, FACS, Differentiation |
| Lineage cocktail | See footnote b | FITC | Biolegend | Progenitors |
| Sca–1 (Ly-6A/E) | D7 (rat IgG2a, κ) | PerCP | Biolegend | Progenitors |
| TER–119 | Ter–119 (rat IgG2b, κ) | BV510, PE-Cy7 | Biolegend | Lineage, FACS |
| Fc Block (CD16/32) | 2.4G2 (rat IgG2b, κ) | Unconjugated | BD Biosciences | BMM |
aExperimental panels refer to: Lineage, bone marrow lineage committed cell populations; FACS, bone marrow myeloid cell sorting by FACS; BMM, cultured bone marrow-derived macrophage immunophenotyping; Differentiation, in vitro myeloid lineage differentiation from Lin- cells; Progenitors, bone marrow hematopoietic progenitor populations.
bLineage cocktail consists of a mixture of FITC-conjugated anti-CD3ε (clone 145-2C11), anti-Gr–1 (clone RB6-8C5), anti-CD11b (clone M1/70), anti-B220 (clone RA3-6B2), and anti-TER–199 (clone Ter–119) and was used according to the manufacturer’s instructions.