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. 2015 Mar;21(3):232–240. doi: 10.1179/2049396714Y.0000000091

Table 1. Primers and enzymes used for PCR–RFLP genotyping.

Gene (polymorphism) ID Primers Restriction enzyme Banding pattern
GSTM1 GAACTCCCTGAAAAGCTAAAGC 219 bp
GTTGGGCTCAAATATACGGTGG
GSTT1 TTCCTTACTGGTCCTCACATCTC 459 bp
TCACCGGATCATGGCCAGCA
ALB GCCCTCTGCTAACAAGTCCTAC 350 bp
GCCCTAAAAAGAAAATCGCCAATC
CYP2E1 -1293 G.>C rs3813867 CAGTCGAGTCTACATTGTC RsaI GG: 410 bp
TTCATTCTGTCTTCTAACTG GC: 410, 290, 120 bp
CC: 290, 120 bp
CYP2E1-1019 C>T rs2031920 CCCGTGAGCCAGTCGAGT PstI CC: 510 bp
ATACAGACCCTCTTCCAC CT: 510, 360, 150 bp
TT: 150, 360 bp
CYP2E1 intro6 T>A rs6413432 CTGCTGCTAATGGTCACTTG HinfI TT: 688 bp
GGAGTTCAAGACCAGCCTAC TA: 688, 350, 338 bp
AA: 350, 338 bp
GSTP1 EX5 A>G rs1695 CTTCCACGCACATCCTCTTCC Alw261 Ile/Ile(AA):289 bp
AAGCCCCTTTCTTTGTTCAGC Val/Val (GG): 218, 71 bp
Ile/Val (AG): 289, 218, 71 bp
mEH EX3–28T>C rs1051740 GATCGATAAGTTCCGTTTCACC EcoR V His/His(TT): 140, 22 bp
ATCCTTAGTCTTGAAGTGAGGAT Tyr/Tyr (CC):162 bp
His/Tyr (CT): 162, 140, 22 bp
mEH EX4+52A>G rs2234922 ACATCCACTTCATCCACGT RsaI His/His (AA):210 bp
ATGCCTCTGAGAAGCCAT Arg/Arg(GG):164, 46 bp
His/Arg (AG): 210, 64, 46 bp