Table 1. Primers and enzymes used for PCR–RFLP genotyping.
Gene (polymorphism) | ID | Primers | Restriction enzyme | Banding pattern |
GSTM1 | – | GAACTCCCTGAAAAGCTAAAGC | – | 219 bp |
GTTGGGCTCAAATATACGGTGG | ||||
GSTT1 | – | TTCCTTACTGGTCCTCACATCTC | – | 459 bp |
TCACCGGATCATGGCCAGCA | ||||
ALB | – | GCCCTCTGCTAACAAGTCCTAC | – | 350 bp |
GCCCTAAAAAGAAAATCGCCAATC | ||||
CYP2E1 -1293 G.>C | rs3813867 | CAGTCGAGTCTACATTGTC | RsaI | GG: 410 bp |
TTCATTCTGTCTTCTAACTG | GC: 410, 290, 120 bp | |||
CC: 290, 120 bp | ||||
CYP2E1-1019 C>T | rs2031920 | CCCGTGAGCCAGTCGAGT | PstI | CC: 510 bp |
ATACAGACCCTCTTCCAC | CT: 510, 360, 150 bp | |||
TT: 150, 360 bp | ||||
CYP2E1 intro6 T>A | rs6413432 | CTGCTGCTAATGGTCACTTG | HinfI | TT: 688 bp |
GGAGTTCAAGACCAGCCTAC | TA: 688, 350, 338 bp | |||
AA: 350, 338 bp | ||||
GSTP1 EX5 A>G | rs1695 | CTTCCACGCACATCCTCTTCC | Alw261 | Ile/Ile(AA):289 bp |
AAGCCCCTTTCTTTGTTCAGC | Val/Val (GG): 218, 71 bp | |||
Ile/Val (AG): 289, 218, 71 bp | ||||
mEH EX3–28T>C | rs1051740 | GATCGATAAGTTCCGTTTCACC | EcoR V | His/His(TT): 140, 22 bp |
ATCCTTAGTCTTGAAGTGAGGAT | Tyr/Tyr (CC):162 bp | |||
His/Tyr (CT): 162, 140, 22 bp | ||||
mEH EX4+52A>G | rs2234922 | ACATCCACTTCATCCACGT | RsaI | His/His (AA):210 bp |
ATGCCTCTGAGAAGCCAT | Arg/Arg(GG):164, 46 bp | |||
His/Arg (AG): 210, 64, 46 bp |