Figure 5.

Estrogen-Induced DNA Strand Breaks Are Dependent on A3B

(A) Hormone-depleted MCF7 cells were used for all experiments. Cells transfected with siA3B or siControl were treated with estrogen. Shown are representative images for γH2AX staining.

(B) The average γH2AX foci number per cell in 100 cells from five replicates ± SEM.

(C) ChIP assay with MCF7 cells transfected with A3B or control siRNA.

(D) Estrogen was added and cells end labeled by incubation with biotin-16-dUTP in the presence of terminal deoxynucleotide transferase (TdT). ChIP was performed with a biotin antibody.

(E and F) Estrogen was added following siRNA transfections for 10 min. Biotin end labeling was performed as in (D).

(C–F) n = 3; ^∗p < 0.001; NS, not significant.