Figure 1.

Human gpr50 is a SIRT1 DNA target. DNA gel electrophoresis is shown of PCR amplicon on unamplified ChIP DNA with DNA primers matching genomic gpr50 at its most 3′exon and downstream non-coding region. The size of this amplicon is about 300 bp. Standard Taq-PCR was performed on the samples indicated. Two independent SIRT1 antibodies were used (lanes 4 and 5).