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. 2015 Oct 8;8:61. doi: 10.3389/fnmol.2015.00061

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Copyright © 2015 Leheste and Torres.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of SIRT1 reverses resveratrol effects on gpr50 expression in HEK-293 cells. (A) Quantitative PCR (QPCR) analysis of resveratrol-induced (50 μM; 48 h) transcriptional changes compared to vehicle only (DMSO). Resveratrol treatment induces significant transcriptional increases in sirt1 (1.9 fold) and gpr50 (5.6 fold). Western-blot analysis supports resveratrol-dependent stimulation of SIRT1 protein levels (inset) (B) QPCR and western blot validation of sirt1 morpholino knockdown from basal levels (columns1–3) and after resveratrol induction (columns 4–6). Values are means ± SD. *Indicates statistical significance of annotated p; n = 20. (C) Morpholino oligonucleotide knockdown of sirt1 reverses resveratrol-induced gpr50 transcriptional increases [5.6 fold→ 1.6 fold (columns 2 and 4)]. The inclusion of a standard control morpholino mix has no significant effect (column 3).