Figure 4.

Increased gpr50 transcription decreases PLC activity in SH-SY5Y cells with a matured dopamine neuronal phenotype. Two ELISA fluorescence readout (λ516 nm) experiments with relevant groups were performed. (A) Whereas melatonin treatment increases PLC activity from baseline by about 10%, luzindole (selective MT1, MT2 antagonist) as well as resveratrol completely reverses this effect. (B) Melatonin-mediated increases in PLC activity are reversed by resveratrol while addition of standard control morpholino mix (CMO) has no significant effect. The resveratrol-effect on PLC activity is reversed by the addition of a SIRT1-specific morpholino (SMO) even in the presence of resveratrol. SIRT1 protein levels (inset) are sensitive to SMO but not CMO. Data are presented as the mean ± SEM. p < 0.05 by Student’s t test for comparison with control (*); n = 12 for all groups.