Fig. 4.

Reduced neurite growth after immune neutralization of neurite growth promoting factors. A, Neutralization of SPARC and PEDF leads to reduced neurite growth of cortical neurons compared with USSC-CM. Neutralization of CST3 had no significant effect on neurite growth. Red arrow: neuron with short neurite; white arrow: long neurite; red arrowhead: nuclei but no neuron, excluded from quantification; white arrowhead: counted cell body of a neuron; interrupted red arrow: artifact excluded from quantification. Neurites labeled by the Neuronal Profiling v3.5 BioApplication software (yellow labeled neurites) were included into quantification. See also enlarged sections for further details. B, Quantification of relative neurite length (%) compared with USSC-CM control. Results derived from at least three independent experiments are presented as mean values ± SEM. * p < 0.05, ** p < 0.01 (Student′s t test).