Table I. Summary of AI-NETD experiments with five different high-pH solvent systems.

Solvent system	5 mm ammonium formate pH 10	5 mm ammonium formate pH 11.5	10 mm piperidine pH ∼ 11.5	5 mm piperidine +5% DMSO, pH ∼ 11.3	5 mm piperidine pH ∼ 11.3
Total MS/MS scans	30,818	32,752	38,856	40,865	39,318
Total peptide spectral matches (PSMs)	10,123	10,371	15,492	16,606	20,099
Total unique peptides	3,659	3,924	5,920	5,208	7,601