Boxplots depicting the agreement of the peptides (A) ELVSEFSR and (b) GLQSLPTHDPSPLQR levels with IHC scores, FISH categories, or HER2 amplification defined according to the ASCO criteria: negative: IHC scores 0+, 1+ and 2+ FISH non amplified; positive: IHC score 3+, 2+, and FISH amplified. The amount of each peptide was adjusted using the appropriate normalization parameter.