Table II. Analytical method precision and whole procedure precision. (A) Analytical method precision calculated for the six HER2 peptides quantified in 40 tumors. (B) Whole procedure precision calculated for the six HER2 peptides from nine of the 40 tumors.
| Peptide sequence | Range peptide amount in tissue extract [pmol] | Reproducibility: Range coefficient of variation [%] |
|---|---|---|
|
A. Analytical method precision (n = 40) | ||
| ELVSEFSR | 0.014–2.73 | 1.1–51.5 |
| FVVIQNEDLGPASPLDSTFYR | 0.026–4.70 | 3.0–58.1 |
| GIWIPDGENVK | 0.009–0.507 | 3.7–52.6 |
| GLQSLPTHDPSPLQR | 0.011–2.25 | 1.3–40.5 |
| SGGGDLTLGLEPSEEEAPR | 0.022–3.11 | 2.4–75.6 |
| SLTEILK | 0.007–2.65 | 0.6–29.9 |
|
B. Whole procedure precision, including sample preparation (n = 9) | ||
| ELVSEFSR | 0.015–0.589 | 16.1–43.6 |
| FVVIQNEDLGPASPLDSTFYR | 0.053–1.22 | 14.7–59.6 |
| GIWIPDGENVK | 0.011–0.141 | 10.2–64.0 |
| GLQSLPTHDPSPLQR | 0.019–0.316 | 12.3–57.1 |
| SGGGDLTLGLEPSEEEAPR | 0.025–0.706 | 18.8–87.0 |
| SLTEILK | 0.010–0.549 | 6.8–31.9 |