Figure 1. Schematic chart showing preparation of fine fractions from D. versipellis and zebrafish screening.

The fractions marked with black caused severe embryonic defect but did not inhibit the outgrowth of ISVs. The fractions marked with blue did not cause a defective phenotype and resulted in normal embryos. The fraction DYVE-D3 marked with red was a positive hit, which inhibited the outgrowth of ISVs specifically. The zebrafish marked with red were screened out for inhibition of ISVs.