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. 2015 Oct 8;5:14468. doi: 10.1038/srep14468

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Copyright © 2015, Macmillan Publishers Limited

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(A) upper row, kaempferol inhibited VEGF-induced HUVECs migration in wound healing assay. HUVECs were allowed to grow into full confluence in 24–well plates. Cells were wounded with a pipette and treated with or without 40 ng/mL VEGF and different concentrations of kaempferol in ECM supplemented with 0.5% (v/v) FBS. Compared to control group, 40 μM kaempferol blocked induction by VEGF. Bottom row, kaempferol inhibited VEGF-induced HUVEC tube formation. (B,C) quantification of the results in (A). Image J was applied for statistical branching point numbers and the distance of remained gap. Scale bar, 100 μm. *P < 0.05 versus control group.