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. 2015 Oct 8;5:14644. doi: 10.1038/srep14644

Figure 2. A PAO1 mutant devoid of the PA4834 gene lost its ability to grow in AMS.

Figure 2

(A) Bacterial strains indicated by numbers were grown in AMS, and the growth index for each strain was measured. Experimental conditions were identical to those described in Fig. 1C. Six independent experiments were performed, and mean values ± SD (error bars) are displayed in each bar. SA, S. aureus. *p < 0.05 vs. growth indices of PA4834::Tn, ΔPA4834, and ΔPA4834/pJN105, as determined by students’ t-test. (B) Scanning electron microscope of primary cultured NHTE cells infected with PAO1, SA and ΔPA4834 mutant. The images were acquired at a magnification of 10,000. (C) Quantitative real-time PCR was performed to assess PA4834, PA4835, PA4836, and PA4837 gene transcript levels. PAO1 cells were grown in LB (gray bars) or AMS (black bars) before RNA extraction. Transcript levels of the tested genes indicated at the bottom were normalized with those of the rpoD gene transcript. Three independent experiments were performed, and mean values ± SD are displayed in each bar. *p < 0.05 vs. expression levels of each gene in LB-grown PAO1, as determined by students’ t-test.