Figure 5. Effect of the addition of nicotianamine (NA) on ΔPA4834 mutant growth in AMS.

PAO1 and the ΔPA4834 mutant were grown in AMS (gray bars) and AMS amended with 100 μM NA (black bars) and growth indices were measured. Experimental conditions were identical to those described in Fig. 1C. Three independent experiments were performed, and mean values ± SD (error bars) are displayed in each bar. *p < 0.05 vs. growth of the ΔPA4834 mutant in AMS with no NA supplementation, as determined by students’ t-test.