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. 2015 Sep 10;162(6):1257–1270. doi: 10.1016/j.cell.2015.08.015

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S6 — Immune-Dependent Control of Growth of Independent Ptgs1/Ptgs2^−/− Clones, Related to Figure 4

(A) WT or Rag1^−/− mice were inoculated with 10⁵ control or two independent Ptgs1/Ptgs2^−/− Braf^V600E melanoma cell lines (sgRNAs2 and sgRNAs3) generated using two combinations of sgRNAs targeting Ptgs1 and Ptgs2 that are different from the combination used to generate the Ptgs1/Ptgs2^−/− Braf^V600E cells used in Figures 2, 3, 4, and 5 (sgRNAs 1). Tumor growth is presented as average tumor diameters ± SEM of three to five mice per group.

(B) The proliferative capacity of the three Ptgs1/Ptgs2^−/− Braf^V600E melanoma cell lines used in this study (sgRNAs 1, sgRNAs 2 and sgRNAs 3) was compared to that of control COX-expressing Braf^V600E cells. The metabolic activity of the cells 72 hr after culture of the indicated number of cells was determined using a non-radioactive cell proliferation assay.