Table 3. The agronomic performance, yield and disease indexes of the transgenic and non-transgenic cotton induced by Verticillium dahliae in field with a history of a high incidence.
| Lines | Height (cm) | No. fruit branch per plant | Boll number of per plant | Single boll weight (g) | Lint percent (%) | Seed cotton yield(kg) | Disease indexes (%) | Dead rate (%) | |
|---|---|---|---|---|---|---|---|---|---|
| Henan | C-19 | 102.2 ± 10.3** | 8.8 ± 0.8** | 11.8 ± 1.6** | 3.52 ± 0.4* | 39.9 ± 1.3* | 141.25 ± 11.6** | 40.9 ± 5.6** | 29.1 ± 4.7** |
| C-26 | 105.6 ± 9.1** | 9.4 ± 1.9** | 12.4 ± 3.6** | 3.32 ± 0.3* | 38.4 ± 1.6 | 143.15 ± 12.9** | 20.6 ± 3.3** | 31.7 ± 1.9** | |
| C-27 | 105.6 ± 7.6** | 9.2 ± 1.2** | 11.4 ± 2.2** | 3.43 ± 0.3* | 38.8 ± 2.5 | 141.05 ± 16.9** | 33.0 ± 4.7** | 18.2 ± 2.5** | |
| C-29 | 104.7 ± 6.6** | 8.6 ± 1.1** | 11.6 ± 1.5** | 3.34 ± 0.2* | 36.5 ± 2.2 | 139.36 ± 11.6** | 32.8 ± 2.9** | 21.4 ± 3.1** | |
| W0 | 86.3 ± 7.5 | 6.5 ± 0.8 | 7.6 ± 1.1 | 2.97 ± 0.4 | 37.5 ± 2.4 | 83.58 ± 9.3 | 65.6 ± 6.3 | 54.8 ± 6.3 | |
| H7124 | 10.7 ± 2.8 | 0.00 | |||||||
| Xinjiang | C-19 | 66.2 ± 5.9** | 9.4 ± 0.58** | 6.4 ± 1.3** | 3.86 ± 0.2* | 43.0 ± 2.0 | 437.5 ± 63.4** | 50.1 ± 8.2** | 31.2 ± 2.9** |
| C-26 | 67.8 ± 3.7** | 9.6 ± 1.0** | 6.0 ± 0.9** | 3.95 ± 0.6* | 44.2 ± 1.9 | 421.9 ± 40.1** | 60.8 ± 6.7** | 35.0 ± 6.1** | |
| C-27 | 65.4 ± 4.2** | 9.2 ± 0.8** | 6.8 ± 0.8** | 4.00 ± 0.4* | 43.2 ± 1.1 | 484.2 ± 41.9** | 36.4 ± 3.4** | 33.6 ± 4.5** | |
| C-29 | 67.4 ± 6.1** | 8.9 ± 1.1** | 6.2 ± 1.1** | 3.78 ± 0.7* | 42.5 ± 2.3 | 417.2 ± 21.9** | 43.8 ± 3.9** | 36.3 ± 2.8** | |
| W0 | 48.5 ± 2.7 | 7.6 ± 0.32 | 4.3 ± 0.8 | 3.26 ± 0.3 | 42.6 ± 2.1 | 249.5 ± 24.9 | 94.6 ± 3.9 | 66.5 ± 7.5 | |
| H7124 | 29.9 ± 2.1 | 0.00 |
The transgenic lines, non-transgenic W0, and the resistance variety G. barbadense cv. Hai7124 were grown at a farm in Henan and Xjinjiang province, China in the 2014 cotton-growing seasons. Seed was planted in a field with a history of a high incidence of Verticillium wilt caused by Verticillium dahliae. Data represent the mean ± SE (n ≥ 15); similar results were obtained from three independent experiments. Plants were scored and classified into five grades as described in our previous report49. The Verticillium wilt disease index was calculated using the following formula: Disease index = [∑ (Ni × i)/(N × 4)] × 100; i = 0 ~ 4, Ni = plant number of reaction I, N is the total number of investigated plants. The analysis of significance was calculated comparing data from W0. Significance of the phenotypic results was assessed using Student’s t tests (*P < 0.05, **P < 0.01).