Supplemental Figure S3.

Histology of longitudinal sections of injured muscles after critical defect muscle injury. Critical defect muscle injury surgery was performed in 8-week-old CD1 mice. Injured muscles were harvested 30 days (A–D) or 60 days (E) after surgery. A–D: Longitudinal sections of injured muscles were stained with Masson's trichrome. Muscle, adipose, and fibrous tissues were stained in red, white, and blue, respectively. Muscle (B), adipose (C), and fibrous (D) tissues were separately selected using Image-Pro software version 7.0. Each tissue was highlighted in green. E: Longitudinal sections stained with hematoxylin and eosin. The initial defect regions are not fully repaired even after 2 months in three independent samples (controls 1 to 3). Scale bars: 0.1 mm (A–E).