Supplemental Figure S5.

Changes in distribution of phosphorylated Smad1/5/8 during muscle repair in the critical defect muscle injury model. Critical defect muscle injury surgery was performed in 8-week-old CD1 mice. Transverse sections of uninjured muscles (A and F) and injured muscles harvested 2 (B, G, and K), 5 (C, H, and L), 14 (D, I, and M), and 30 (E, J, and N) days after surgery were stained with anti–phospho-Smad1/5/8. F–N are magnified images of the indicated areas in A–E. Arrowheads in F indicate phospho-Smad1/5/8–positive satellite cells. Arrows in G indicate coagulated area. Arrowheads in M and N indicate phospho-Smad1/5/8–positive immature muscle fibers with central nuclei. Scale bars: 100 mm (A–E); 50 mm (F–N).