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. 2015 Sep;185(9):2390–2401. doi: 10.1016/j.ajpath.2015.05.015

Figure 5.

Figure 5

Levels of activated activator protein 1 and NF-κB remain largely unchanged in neonatal brain in response to upper-uterine injection of lipopolysaccharide (LPS) serotype. Whole neonatal brain tissue extracts, collected 6 hours after intrauterine injection of either phosphate-buffered saline (PBS) or LPS serotypes O111:B4, O55:B5, O127:B8, or O128:B12, were assessed by Western blot analysis for p-c-Jun (A and B) and p-p65 (A and C). No changes in inflammatory transcription factor activation in response to PBS or LPS administration are observed at 6 hours after treatment. Densitometric analysis of immune-reactive bands was performed relative to β-actin (one-way analysis of variance with Tukey's post hoc test). n = 5 (A–C). AU, arbitrary unit; CAL, calibrator; C/EBP, CCAAT/enhancer-binding protein; Ctrl, control; LAP, liver-enriched activator protein; LIP, liver-enriched inhibitory protein.