Figure 3.

Macrophage and microglia infiltration after demyelination in glial fibrillary acidic protein (GFAP)-signal transducer and activator of transcription 3 (STAT3)-CKO mice is similar to that in controls. The microglia/macrophages in the demyelinated area at 5 days after lesion (dpl) were visualized with antibody against macrophage/microglia marker ionized calcium binding adaptor molecule 1 (Iba1) in control (A) and GFAP-STAT3-CKO (B) mice. C: Mean optical density was used to compare the Iba1 immunoreactivity between controls and mutants across time points. D: The average macrophage/microglia infiltration area at different survival times. Measurements were taken from transverse sections of the lesion that had the largest lesion area (identified by increased cellularity). Dotted lines mark the lesion boundaries. Means ± SEM are shown. Scale bar = 50 μm (B, applies to all images).