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. 2015 Sep;185(9):2431–2440. doi: 10.1016/j.ajpath.2015.05.011

Figure 6.

Figure 6

Schwann cell remyelination increases in glial fibrillary acidic protein (GFAP)-signal transducer and activator of transcription 3 (STAT3)-CKO mice. Remyelinating Schwann cells from control and GFAP-STAT3-CKO mice at 21 days after lesion (dpl) were examined by periaxin immunostaining (A–D) and myelin protein zero (mpz) mRNA in situ hybridization (F–H). Dotted lines (A–D, F, and G) mark demyelinated areas. The inset in C shows magnified boxed area with periaxin and Hoechst 33342. E and I: Quantification of the relative area of positive periaxin immunofluorescence (E) and cells containing mpz mRNA (I). J and K: Semithin resin sections from control (J) and STAT3CKO (K) lesioned mice were stained with toluidine blue. Areas of oligodendrocyte remyelination are marked with the yellow letter O, and that of Schwann cells are marked with the red letter S. M and N: Enlarged boxed areas from panels J and K, respectively. L and O: Examples of axons remyelinated by oligodendrocytes are marked by yellow arrowheads; green arrowheads point to examples of axons that were not demyelinated, blue arrowheads indicate poorly remyelinated axons, and red arrowheads mark typical morphology of myelinating Schwann cells. These observations were verified further by electron microscopy with examples shown in control (L) and GFAP-STAT3-CKO (O) animals. Inset (O) shows an enlarged view of the boxed area, showing the typical structure of myelinating Schwann cells. Dotted lines mark the lesion boundaries. Means ± SEM are shown. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. Scale bars: 100 μm (A–D, F, G, J–M); 5 μm (N and O).