Figure 3.

Active Jak2-Stat5a/b signaling induces epithelial-to-mesenchymal transition marker expression in clinical prostate cancers (PCs) ex vivo in organ explant cultures. A–C: Five patient-derived PCs (Table 1) were cultured ex vivo in explant organ cultures for 7 days in the presence of AZD1480 versus vehicle. AZD1480 decreases nuclear Stat5a/b immunostaining (A) in a dose-dependent manner, whereas it increases cell surface E-cadherin (E-Cad) expression (B) and decreases Twist1 expression (C) in clinical PCs. Statistical significance was calculated using a linear mixed-effects model with empirical SEMs. ^∗P < 0.05, ^∗∗∗P < 0.001.