Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Schematic of steps required for inserting a cDNA of interest into pRosa26-DEST. A cDNA is amplified using PCR to add In-Fusion (IF) adapter sequences. The In-Fusion reaction is then performed between the PCR product and linearized Entry vector to obtain a Gateway-ready Entry vector containing the cDNA. This vector is then combined with pRosa26-DEST in a LR reaction to obtain to targeting vector.
