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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Curr Protoc Mouse Biol. 2015 Jun 1;5(2):187–204. doi: 10.1002/9780470942390.mo140150

Table 1.

Primer List

Primer Sequence (5′ – 3′)
ROSA26 PCR screen forward GGCGGACTGGCGGGACTA
ROSA26 PCR screen reverse GGGACAGGATAAGTATGACATCATCAAGG
ROSA26 5′ Southern probe F GGCTCCTCAGAGAGCCTC
ROSA26 5′ Southern probe R CCGGCTGTCTCACAGAAC
ROSA26 3′ Southern probe F ACTTCCCACAGATTTTCGGTT
ROSA26 3′ Southern probe R TCTCAAGCAGGAGAGTATAAAACTC