Table 1.
Primer | Sequence (5′ – 3′) |
---|---|
ROSA26 PCR screen forward | GGCGGACTGGCGGGACTA |
ROSA26 PCR screen reverse | GGGACAGGATAAGTATGACATCATCAAGG |
ROSA26 5′ Southern probe F | GGCTCCTCAGAGAGCCTC |
ROSA26 5′ Southern probe R | CCGGCTGTCTCACAGAAC |
ROSA26 3′ Southern probe F | ACTTCCCACAGATTTTCGGTT |
ROSA26 3′ Southern probe R | TCTCAAGCAGGAGAGTATAAAACTC |