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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Anticancer Res. 2015 Oct;35(10):5249–5261.

Figure 3.

Figure 3

Vascular Endothelial Growth Factor (VEGF)165, ATP-binding cassette sub-family B member 1 (ABCB1), Glucose Transporter 1 (GLUT1), Phosphoglycerate Kinase (PGK1) and Carbonic Anhydrase IX (CAIX) mRNA expression in MCF-7 and MDA-MB-231 cells. MCF-7 cells and MDA-MB-231 cells were seeded in T25 flasks, and treated with 0.5 μM STX140, STX243 or STX66, or with 5 μM STX66 under normoxia for 18 h and under hypoxia (1% O2, 5% CO2) for 6 h. Total mRNA was then prepared, reverse-transcribed and the relative quantity of VEGF165, ABCB1, GLUT1, PGK1 and CAIX were determined by RT-PCR. mRNA expression of untreated cells after 6 h hypoxia of each experiment was given the value 1. *p<0.05, **p<0.01, ***p<0.001, compared to control (Ct).