Table 1.

Nucleotide sequences of primers used in real-time PCR

Gene (accession no.)	Primes	Nucleotiede sequences5‘-3’	Size(bp)	Temp(°C)
EP1	Forward	CTGCGTCATCCATCACTT	165	55.0
	Reverse	CCAACACCACCAATACCA		55.0
EP2	Forward	CGGATTGTCTGGCAGTAG	124	57.3
	Reverse	GCATACAGCGAAGGTGAT		55.0
EP3	Forward	CTTCAATCAGATGTCAGTAGAG	181	57.8
	Reverse	CCGCTTCAGGTTGTTCAT		55.4
EP4	Forward	CTATACCTGCCAGACCTAAC	174	56.3
	Reverse	CTCATCCACCAACAAGACA		57.3
GAPDH	Forward	TTCAACGGCACAGTCAAG	116	55.0
	Reverse	TACTCAGCACCAGCATCA		55.0