Table 1.
Data collection.
| CPV4 Cell 1 | CPV4 Cell 2 | CPV5 | CPV5 SeMet | CPV14 | CPV14 SeMet | CPV15 | CPV15 SeMet | CPV17 room temp | CPV17 frozen | CPV18 | CPV19 | CPV19 SeMet | CPV20 | CPV1Δ | CPV4Δ | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number of crystals | 18 | 11 | 26 | 128 | 20 | 13 | 40 | 112 | 5787 | 768 | 21 | 34 | 61 | 51 | 13 | 21 |
| Crystal size (μm) | 5–7 | 5–7 | 3–4 | 3–4 | 3–4 | 3–4 | ∼1.5 | ∼1.5 | ∼1.0 | ∼1.0 | 4–5 | ∼3 | ∼3 | 3–4 | 4–6 | 5–7 |
| Cell a = b = c (Å) | 101.7 | 104.8 | 102.3 | 102.1 | 102.6 | 102.8 | 102.9 | 103.5 | 106.1 | 104.9 | 102.8 | 102.7 | 103.3 | 103.4 | 103.9 | 104.3 |
| Resolution (Å)a | 29.4–1.72 (1.82–1.72) |
28.0–2.00 (2.11–2.00) |
51.2–1.61 (1.65–1.61) |
72.2–2.07 (2.14–2.07) |
72.5–1.91 (2.02–1.91) |
36.3–2.24 (2.36–2.24) |
51.4–1.82 (1.86–1.82) |
51.7–2.48 (2.55–2.48) |
28.3–1.75 (1.79–1.75) |
74.16–2.20 (2.26–2.20) |
72.7–1.48 (1.51–1.48) |
51.4–1.50 (1.59–1.50) |
42.1–2.03 (2.08–2.03) |
51.7–1.82 (1.87–1.82) |
42.4–1.88 (1.93–1.88) |
33.0–1.71 (1.75–1.71) |
| Unique reflections | 17233 (1580) |
11543 (797) |
23283 (1726) |
10902 (1093) |
12952 (1045) |
7956 (632) |
16418 (1193) |
6666 (473) |
19096 (1032) |
9376 (931) |
30415 (2238) |
21653 (498) |
11977 (870) |
16582 (1219) |
15023 (1093) |
19985 (1319) |
| Rmerge | 0.199 (0.385) |
0.177 (0.330) |
0.342 (0.000)⁎⁎ |
0.163⁎ (0.387⁎) |
0.199 (0.327) |
0.186 (0.363) |
0.468 (0.000)⁎⁎ |
0.499 (0.000)⁎⁎ |
0.665 (0.000) |
0.320 (0.000)⁎⁎ |
0.368 (0.000)⁎⁎ |
0.437 (0.000)⁎⁎ |
0.342 (0.000)⁎⁎ |
0.226 (0.000)⁎⁎ |
0.189 (0.000)⁎⁎ |
|
| I/σI | 9.7 (1.7) |
8.1 (1.7) |
7.0 (1.4) |
7.5 (1.9) |
8.4 (1.8) |
7.1 (1.6) |
4.2 (0.8) |
6.4 (1.8) |
6.4 (1.4) |
7.1 (1.5) |
3.8 (0.3) |
5.4 (1.0) |
5.4 (1.1) |
4.8 (1.0) |
5.0 (0.6) |
|
| Completeness (%) | 92.7 (59.0) |
88.5 (42.7) |
100 (100) |
100 (100) |
92.3 (52.1) |
90.0 (50.3) |
99.7 (97.9) |
100 (100) |
100 (100) |
99.9 (100) |
100 (99.8) |
75.5 (12.2) |
98.7 (91.5) |
100 (99.7) |
98.8 (98.6) |
97.3 (88.9) |
| Redundancy | 11.4 (1.9) |
7.6 (1.5) |
14.8 (10.7) |
64.1 (26.1) |
7.9 (1.6) |
6.7 (1.5) |
7.8 (4.5) |
19.8 (13.3) |
52.4 (11.1) |
47.8 (25.4) |
12.9 (8.6) |
3.8 (1.1) |
14.3 (6.4) |
10.0 (6.5) |
4.3 (4.0) |
4.4 (2.7) |
CPV17 from (Ginn et al., 2015b).
a
Values in parentheses are for the highest resolution shell.
⁎
Rmerge obtained by scaling of two already processed data sets.
⁎⁎
Rmerge> = 1.