Table 2.

PCR primers used in this study.

Target gene	Nucleotide sequence (5′–3′)	Annealing temperature (°C)	Product size (bp)	References
blaTEM	F: CATTTCCGTGTCGCCCTTATTC R: CGTTCATCCATAGTTGCCTGAC	58	800	Luo et al., 2011
blaSHV	F: AGCCGCTTGAGCAAATTAAAC R: ATCCCGCAGATAAATCACCAC	58	713	Luo et al., 2011
blaCTX−M−1 group	F: TTAGGAARTGTGCCGCTGYAa R: CGATATCGTTGGTGGTRCCATa	55	688	Luo et al., 2011
blaCTX−M−2 group	F: CGTTAACGGCACGATGAC R: CGATATCGTTGGTGGTRCCATa	55	404	Luo et al., 2011
blaCTX−M−9 group	F: TCAAGCCTGCCGATCTGGT R: TGATTCTCGCCGCTGAAG	58	561	Luo et al., 2011
blaCTX−M−8∕25 group	F: AACRCRCAGACGCTCTACa R: TCGAGCCGGAASGTGTYATa	55	326	Luo et al., 2011
qnrA	F: TCAGCAAGAGGATTTCTCA R: GGCAGCACTATTACTCCCA	58	627	Robicsek et al., 2005
qnrB	F: GATCGTGAAAGCCAGAAAGC R: ACGATGCCTGGTAGTTGTCC	53	469	Gay et al., 2006
qnrS	F: ACGACATTCGTCAACTGCAA R: TAAATTGGCACCCTGTAGGC	53	417	Gay et al., 2006
qnrC	F: GGGTTGTACATTTATTGAATC R: TCCACTTTACGAGGTTCT	52	447	Wang et al., 2009
qnrD	F: CGAGATCAATTTACGGGGAATA R: AACAAGCTGAAGCGCCTG	52	582	Cavaco et al., 2009
aac(6')-Ib	F: TTGCGATGCTCTATGAGTGGCTA R: CTCGAATGCCTGGCGTGTTT	55	482	Park et al., 2006
qepA	F: AACTGCTTGAGCCCGTAGAT R: GTCTACGCCATGGACCTCAC	55	596	This study
oqxA	F: GCGTCTCGGGATACATTGAT R: GGCGAGGTTTTGATAGTGGA	56	482	This study
oqxB	F: CTGGGCTTCTCGCTGAATAC R: CAGGTACACCGCAAACACTG	55	498	This study
intI1	F: CCTCCCGCACGATGATC R: TCCACGCATCGTCAGGC	54	280	Dillon et al., 2005
intI2	F: AAATCTTTAACCCGCAAACGC R: ATGTCTAACAGTCCATTTTTAAATTCTA	54	439	Dillon et al., 2005
intI3	F: AGTGGGTGGCGAATGAGTG R: TGTTCTTGTATCGGCAGGTG	54	599	Dillon et al., 2005
IntI1 variable region	F: TCATGGCTTGTTATGACTGT R: GTAGGGCTTATTATGCACGC	56	Variable	White et al., 2000

^aY = T or C; R = A or G; S = G or C; D = A or G or T.

F, forward; R, reverse.