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. Author manuscript; available in PMC: 2016 Jul 7.
Published in final edited form as: Cell Metab. 2015 Jun 18;22(1):77–85. doi: 10.1016/j.cmet.2015.05.021

Figure 4. DMB increases human β-cell proliferation in vitro and in vivo.

Figure 4

(A) Percent BrdU-positive β-cells in human islet cell cultures treated with Veh (−) or different concentrations of DMB, and BrdU for 24h. (B) Human islet cell cultures treated with Veh or 0.1μg/ml of DMB for 24h, and stained for insulin (red), Ki67 (green), and DAPI (blue). (C) Percent Ki67-positive human β-cells treated with Veh (−) (as 100%) or 0.1μg/ml of DMB for 24 or 72h; (n=3-5 human islet preps). (D) Experimental design of human islets transplanted under the kidney capsule of male euglycemic NOD/SCID mice. (E) Representative images of human islet grafts in the kidney capsule of Veh or DMB (1μg/g) treated mice stained for insulin (green) and BrdU (red). Arrow represents BrdU-positive β-cell. (F) Percent BrdU-positive β-cells in human islet grafts of Veh and DMB-treated mice (n=5 human islet preps; 1427±121 β-cells counted/prep). All values are presented as mean ± SEM. *p<0.05 vs Veh.