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| Item | Condition |
|---|---|
| Jasco Co., Japan | |
| Pump : PU-980 | |
| HPLC | Detector : UV-975 |
| Column oven : CO-965 | |
| Auto sampler : AS-2057 | |
| Column | Shiseido C18 (4.6×205 mm, 5 μm) |
| A: 100% Methanol | |
| Mobile phase | B: 0.25M Ammonium acetate (pH 5.5) |
| with acetic acid | |
| Temperature | 40℃ |
| Velocity | 1.5 mL/min |
| Detector | UV detector |
| Wavelength | 254 nm |
