Table 1. Bacterial bioluminescent bioreporter for organic contaminants and representative environmental applications.

Reporter strain	Reporter construct	Target compound	Detection limit	References	Environmental application
E. coli DH5α (pPROBE-LuxAB-TubT)	tubT-luxAB	BTEX	0.24 μM toluene	[14]	Simulated aquatic oil spill [14]
E. coli DH5α pGLTUR	xylR-Pu-luc	Benzene, Toluene, Xylene	10–20 μM toluene 3 μM 3-xylene	[13]	Soil [13, 131]
P. putida TVA8	tod-luxCDABE	BTEX	0.03–50 ppm BTEX	[15]	Soil [3]
		TCE	1 μMTCE		Water [18]
E. coli HMS174 (pOS25)	ipbR-luxCDABE	Hydrophobic compounds	1 μM toluene 0.1 μM naphthalene	[22]	Soil [132]
E. coli DH5α pTOLLUX	pR/xlyR-Pu-luxCDABE	BTEX	N/A	[11]	Soil and groundwater [11]
B. sartisoli RP007 (pPROBE-phn-luxAB)	phnS-luxAB	PAH	0.17 μM naphthalene	[14]	Simulated aquatic oil spill [14]
P. putida pPG7-JAMA21	nahR-Psal-luxAB	Naphthalene	0.50 μM naphthalene vapor	[133]	N/Aa
P. fluorescens HK44 (pUTK21)	nahR-PnahG-luxCDABE	PAH	12–120 μM	[29]	Soil [32, 131, 134]
E. coli DH5 (pGEc74, pJAMA7)	alkS-PalkB-luxAB	6–10-carbon alkane	25 nM octane	[37]	Groundwater [20] Soil [131]
E. coli	alkBFG, alkJ-luxAB	5–12-carbon alkane	10 μM octane	[39]	N/A
Acinetobacter baylyi ADP1	alkR-PalkM- luxCDABE	Alkane	3 nM octane	[43]	Seawater [43]
E. coli	alkR-PalkM-luxAB	Alkane	5 nM octane	[45]	N/A
P. putida F1G4	sep-luxCDABE	TCE	1 mM TCE	[50]	N/A
Methylobacterium extorquens	dcmR-PdcmA-luxCDABE	DCM	1 μM DCM	[51]	N/A
Ralstonia eutropha ENV307 (pUTK60)	bphR1-luxCDABE	PCBs	0.80 μM 4-chlorobiphenyl 4.6 μM Aroclor	[53]	N/A
E. coli XL1-Blue	PpcbC-luxCDABE	PCBs	0.1 mM biphenyl (lowest concentration tested)	[56]	N/A
E. coli XL1-Blue	PpcbC-luc	PCBs	0.1 mM biphenyl (lowest concentration tested)	[56]	N/A
E. coli (pHYBP109)	hbpR-PhbpC-luxAB	OH-PCBs	1 nM 2-hydroxy-3′,4′,5-trichlorobiphenyl	[58]	Simulated aquatic oil spill [14] Human serum [58]
P. fluorescens OS8(pDNdmpRlux)	dmpR-Po-luxCDABE	Phenolic compound	0.30 μM 2-methylphenol 0.87 μM phenol	[63]	Groundwater and semicoke-dump leachates [63]
E. coli (pRLuc42R)	luc	Phenolic compound	0.5 μM phenol	[65]	N/A
Acinetobacter DF4-8	mopR-luxCDABE	Phenolic compound	0.03 mM phenol	[67]	N/A

^aN/A not available