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. 2015 Oct 8;10(10):e0139520. doi: 10.1371/journal.pone.0139520

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© 2015 Meyer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Chk1, (B) Emi1/Fbxo5, (C) Mybl2 protein were detected by western blot analysis. Histone H3 served as the normalization control. Murine mouse muscle cells were cultured for 48 h in growth medium (lane 1), differentiation medium (lane 2), or differentiation medium supplemented with TNF-α (lane 3) or IGF1 (lane 4), respectively. (B) The specificity of the double band between 40 and 50 kDa was confirmed by peptide competition of the Emi/Fbxo5 antibody epitope (S4 Fig).