Table 2. Genes describing the treatment effects.
| 4 h myoblast differentiation: control, TNF-α or IGF1 treatment | |
| Gene symbol | Gene title |
| Tmeff2 | transmembrane protein with EGF-like and two follistatin-like domains 2 |
| Sdpr | serum deprivation response |
| Nrp2 | neuropilin 2 |
| Fam78b | family with sequence similarity 78, member B |
| Sh2d1b1 | SH2 domain protein 1B1 |
| Pkp1 | plakophilin 1 |
| Ctgf | connective tissue growth factor |
| Etv5 | ets variant gene 5 |
| Chst11 | carbohydrate sulfotransferase 11 |
| Aldh3a1 | aldehyde dehydrogenase family 3, subfamily A1 |
| Etv4 | ets variant gene 4 (E1A enhancer binding protein, E1AF) |
| Lpin1 | lipin 1 |
| Id2 | inhibitor of DNA binding 2 |
| Pgf | placental growth factor |
| Itgb8 | integrin beta 8 |
| Idi1 | isopentenyl-diphosphate delta isomerase |
| Serpinb6b | serine (or cysteine) peptidase inhibitor, clade B, member 6b |
| Rbm24 | RNA binding motif protein 24 |
| Serpinb1a | serine (or cysteine) peptidase inhibitor, clade B, member 1a |
| Slc22a23 | solute carrier family 22, member 23 |
| Tmem171 | transmembrane protein 171 |
| Hmgcs1 | 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 |
| Sema5a | sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A |
| Vdr | vitamin D receptor |
| Sp7 | Sp7 transcription factor 7 |
| Etv5 | ets variant gene 5 |
| Robo2 | roundabout homolog 2 (Drosophila) |
| Smad7 | MAD homolog 7 (Drosophila) |
| Hbegf | heparin-binding EGF-like growth factor |
| Sema6a | sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A |
| Dusp5 | dual specificity phosphatase 5 |
| Syt12 | synaptotagmin XII |
| Dlx1 | distal-less homeobox 1 |
| Ak4 | adenylate kinase 4 |
| Id1 | inhibitor of DNA binding 1 |
| Qrfp | pyroglutamylated RFamide peptide |
| Idi1 | isopentenyl-diphosphate delta isomerase |
| Dlx2 | distal-less homeobox 2 |
| Ccdc141 | coiled-coil domain containing 141 |
| Jag1 | jagged 1 |
| Fgf2 | fibroblast growth factor 2 |
| Hspa4l | heat shock protein 4 like |
| Smad9 | MAD homolog 9 (Drosophila) |
| Prr9 | proline rich 9 |
| Lce1g | late cornified envelope 1G |
| Lphn2 | latrophilin 2 |
| Gm12824 | predicted gene 12824 |
| Id3 | inhibitor of DNA binding 3 |
| Sema3d | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D |
| Lrrc17 | leucine rich repeat containing 17 |
| Ereg | epiregulin |
| Hk2 | hexokinase 2 |
| C3ar1 | complement component 3a receptor 1 |
| Dyrk1b | dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1b |
| Pdlim3 | PDZ and LIM domain 3 |
| Cx3cl1 | chemokine (C-X3-C motif) ligand 1 |
| Foxc2 | forkhead box C2 |
| Sc4mol | sterol-C4-methyl oxidase-like |
| 1600029D21Rik | RIKEN cDNA 1600029D21 gene |
| Ubash3b | ubiquitin associated and SH3 domain containing, B |
| Smad6 | MAD homolog 6 (Drosophila) |
| 12 h myoblast differentiation: control, TNF-α or IGF1 treatment | |
| Gene symbol | Gene title |
| miR-206 | microRNA-206-3p |
| miR-133b | microRNA-133b-3p |
| Myog | myogenin |
| Selp | selectin, platelet |
| Olfml2b | olfactomedin-like 2B |
| Lgr6 | leucine-rich repeat-containing G protein-coupled receptor 6 |
| Fabp7 | fatty acid binding protein 7, brain |
| Idi1 | isopentenyl-diphosphate delta isomerase |
| Rbm24 | RNA binding motif protein 24 |
| Stc1 | stanniocalcin 1 |
| Sntb1 | syntrophin, basic 1 |
| Sp7 | Sp7 transcription factor 7 |
| Adamts5 | a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) |
| Dll1 | delta-like 1 (Drosophila) |
| Dtx4 | deltex 4 homolog (Drosophila) |
| Slc24a3 | solute carrier family 24 (sodium/potassium/calcium exchanger), member 3 |
| Id1 | inhibitor of DNA binding 1 |
| Idi1 | isopentenyl-diphosphate delta isomerase |
| Dlx2 | distal-less homeobox 2 |
| Ttn | titin |
| Ccdc141 | coiled-coil domain containing 141 |
| Fibin | fin bud initiation factor homolog (zebrafish) |
| Slc7a11 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 |
| Gm12824 | predicted gene 12824 |
| Car8 | carbonic anhydrase 8 |
| Dync1i1 | dynein cytoplasmic 1 intermediate chain 1 |
| Cpa1 | carboxypeptidase A1 |
| Cdkn1c | cyclin-dependent kinase inhibitor 1C (P57) |
| Sc4mol | sterol-C4-methyl oxidase-like |
| 24 h myoblast differentiation: control, TNF-α or IGF1 treatment | |
| Gene symbol | Gene title |
| Car8 | carbonic anhydrase 8 |
| Ccdc141 | coiled-coil domain containing 141 |
| Cdkn1c | cyclin-dependent kinase inhibitor 1C (P57) |
| Cpa1 | carboxypeptidase A1 |
| Ctrb1 | chymotrypsinogen B1 |
| Dlx2 | distal-less homeobox 2 |
| Dtx4 | deltex 4 homolog (Drosophila) |
| Dync1i1 | dynein cytoplasmic 1 intermediate chain 1 |
| Erbb3 | v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) |
| Fabp7 | fatty acid binding protein 7, brain |
| Hfe2 | hemochromatosis type 2 (juvenile) (human homolog) |
| Lgr6 | leucine-rich repeat-containing G protein-coupled receptor 6 |
| miR-133b | microRNA-133b-3p |
| miR-206 | microRNA-206-3p |
| Myog | myogenin |
| Olfml2b | olfactomedin-like 2B |
| Slc24a3 | solute carrier family 24 (sodium/potassium/calcium exchanger), member 3 |
| Slc7a11 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 |
| Sntb1 | syntrophin, basic 1 |
| Stc1 | stanniocalcin 1 |
| Ttn | titin |
Nonambigous genes identified by dynamic principal component analysis (group selection myoblasts) of gene expression profiling data after 4 h, 12 h, or 24 h of differentiation or TNF-α or IGF1 treatment identified genes that were sufficient for separation of treatment groups by principal components as shown in Fig 2C, 2F and 2I.