Table 1. Primer information for the 8 genes examined with relative qPCR.

Gene Symbol	Gene Name	Primer Sequence	Amplicon Size (bp)	Tm (°C)	Cycling Parameters
		(+ sense and–antisense)
Comt	Catechol o-methyltransferase	(+) atcttcacggggtttcagtg	145	60.0
		(-) gagctgctggggacagtaag
Drd2	Dopamine receptor D2	(+) gccgagttactgtcatgattgc	154	60.0
		(-) ggcacgtagaatgagacaatgg
Drd3	Dopamine receptor D3	(+) tcttcagtggtgtccttctacg	200	59.0
		(-) ctggcccttattgaaaactgcc
Drd4	Dopamine receptor D4	(+) cctcaaccccatcatctacacc	193	60.0	1 cycle 95°C 3 min,
		(-) cccagcgttgataaatggttagg
Maoa	Monoamine oxidase A	(+) gagaagaactggtgtgaggagc	170	60.0	40 cycles 95°C 15 sec,
		(-) tcaactgctccttccatgtagc
SERT	Serotonin transporter (Slc6a4)	(+) gtggactctcagacatgctacc	190	60.0	40 cycles 95°C 15 sec,
		(-) ttagaagctaacagatgcgggg
Tph1	Tryptophan hydroxylase 1	(+) cactcactgtctctgaaaacgc	216	60.0	40 cycles Tm °C 30 sec,
		(-) agccatgaatttgagagggagg
Tph2	Tryptophan hydroxylase 2	(+) gtgtgtaaaagcctttgacccg	181	60.0	+Melt Curve
		(-) ttcaatgctctgtgtgtagggg
CycA	Cyclophilin A	(+) agcactggggagaaaggatt	248	58.0
		(-) agccactcagtcttggcagt
Ywhaz	Tyrosine 3-monooxygenase/ tryptophan, 5-monooxygenase activation protein, zeta	(+) ttgagcagaagacggaaggt	136	56.1
		(-) gaagcattggggatcaagaa