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. Author manuscript; available in PMC: 2015 Oct 8.
Published in final edited form as: Biomaterials. 2011 Mar 22;32(19):4450–4463. doi: 10.1016/j.biomaterials.2011.02.059

Fig. 1.

Fig. 1

HPMA copolymer-ALN-TNP-470 conjugate is hemocompatible and does not cause leukopenia in non-tumor bearing mice. (A) Red Blood Cells Lysis (RBC) assay of free or conjugated ALN and TNP-470. HPMA copolymer-ALN-TNP-470 conjugate (closed triangles) did not induce hemolysis at all tested concentrations compared with free ALN plus TNP-470 (open triangles) that induced hemolytic effect at concentrations higher than 1 mg/ml. Positive control of PEI (closed squares) showed dose dependent hemolysis. Results are presented as % of hemoglobin release ± s.d. (B) Effect of free or conjugated ALN and TNP-470 on peripheral WBC counts in Balb/c mice. Free combination of ALN plus TNP-470 had insignificant effect on WBC levels similar to HPMA copolymer-ALN-TNP-470 conjugate as compared with mice treated with vehicle (control group). Data represent mean ± s.e.m.