Table 2. Fatty acids, sphingomyelins, and eicosanoids showing significant differences between the different Wrn mutants and WT mice.

Metabolites concentrations higher in both Wrn Δhel/Δhel and Wrn -/- compared to WT mice
Metabolites	WT levels	Wrn Δhel/Δhel levels	Wrn -/- levels	ANOVA
lysoPC a C26:0	0.14 ± 0.04	0.41 ± 0.24	0.47 ± 0.18	0.0110
lysoPC a C26:1	0.07 ± 0.02	0.20 ± 0.14	0.22 ± 0.08	0.0304
lysoPC a C28:0	0.18 ± 0.02	0.45 ± 0.16	0.45 ± 0.12	0.0009
lysoPC a C28:1	0.14 ± 0.02	0.42 ± 0.27	0.54 ± 0.22	0.0114
PC ae C30:0	0.16 ± 0.01	0.29 ± 0.10	0.27 ± 0.06	0.0161
PC ae C30:1	0.10 ± 0.03	0.18 ± 0.08	0.20 ± 0.07	0.0298
PC ae C30:2	0.07 ± 0.01	0.12 ± 0.03	0.13 ± 0.02	0.0003
PC ae C36:0	0.44 ± 0.05	0.69 ± 0.23	0.63 ± 0.06	0.0178
PC ae C36:1	2.72 ± 0.33	4.09 ± 1.06	4.72 ± 0.51	0.0007
PC ae C38:1	1.05 ± 0.11	1.76 ± 0.37	2.08 ± 0.35	0.0001
PC aa C28:1	0.13 ± 0.02	0.35 ± 0.13	0.61 ± 0.08	0.0001
Metabolites concentrations higher in Wrn -/- mice than in both Wrn Δhel/Δhel and WT mice
Metabolites	WT levels	Wrn Δhel/Δhel levels	Wrn -/- levels	ANOVA
lysoPC a C16:0	192 ± 28	180 ± 18	272 ± 38	0.0001
lysoPC a C16:1	6.14 ± 1.59	5.25 ± 1.25	9.72 ± 3.12	0.0071
lysoPC a C17:0	2.67 ± 0.49	3.02 ± 0.33	4.07 ± 0.47	0.0001
lysoPC a C18:1	43.7 ± 10.2	37.0 ± 8.3	62.1 ± 12.3	0.0023
lysoPC a C18:2	160 ± 23	148 ± 16	224 ± 25	0.0001
lysoPC a C20:3	10.1 ± 4.3	8.8 ± 3.7	14.9 ± 3.2	0.0358
PC ae C40:5	1.06 ± 0.11	1.13 ± 0.15	1.72 ± 0.07	0.0001
PC ae C44:5	0.18 ± 0.02	0.19 ± 0.04	0.32 ± 0.05	0.0001
PC aa C24:0	0.06 ± 0.01	0.10 ± 0.06	0.14 ± 0.07	0.0462
PC aa C34:2	349 ± 40	366 ± 38	358 ± 58	0.0001
SM C20:2	0.02 ± 0.02	0.03 ± 0.03	0.11 ± 0.04	0.0004
PGE2	44.3 ± 20.0	27.5 ± 20.0	124.2 ± 86.7	0.0142

Values are in μM ± SD except for PGE2 which is in nM.