Fig 3. Growth on arabinogalactan induces the secretion of pNP-β-l-Arabinopyranosidase activity.

(A) Hydrolysis of pNP-α-d-Galp (solid) and pNP-β-l-Arap (striped) by secretomes induced by different growth carbohydrates. (B) pNP-β-l-arabinopyranosidase activity is more strongly enriched in the secreted protein fraction than other cellular locations, and is much more significant in cultures induced by arabinogalactan (striped) compared to cultures induced by glucose (solid). The term ‘associated’ denotes proteins which are weakly associated with cell membranes. Abbreviations are as follows: Glc, glucose; Gal, galactose; CGM, carob galactomannan; LAG, larch wood arabinogalactan; GAR, gum arabic.