Table 2. Kinetic parameters of different AChEs.
| Substrate | Vmax (mA/min) | Km (μM) | Vmax/KM | Kcat (s-1) |
|---|---|---|---|---|
| Aedes aegypti AChE1 | ||||
| ATChI | 406 (389–422) | 25 (20–30) | 16.2 | 140 |
| PTChI | 266 (258–275) | 20 (17–23) | 13.3 | 92 |
| BTChI | 38 (36–40) | 45 (36–54) | 0.8 | 13 |
| Anopheles gambiae AChE1 | ||||
| ATChI | 528 (505–552) | 27 (21–33) | 19.6 | 124 |
| PTChI | 407 (393–421) | 25 (20–29) | 16.3 | 95 |
| BTChI | 58 (55–61) | 36 (27–45) | 1.6 | 14 |
| Anopheles gambiae AChE1-G119S | ||||
| ATChI | 318 (310–326) | 58 (52–65) | 5.5 | n. d. |
| PTChI | 105 (87–123) | 303 (172–435) | 0.27 | n. d. |
| BTChI | 34 (24–45) | 431 (136–727) | 0.08 | n. d. |
| Mus musculus AChE | ||||
| ATChI | 725 (694–756) | 84 (72–95) | 8.6 | n. d. |
| PTChI | 367 (354–380) | 59 (51–66) | 6.2 | n. d. |
| Homo sapiens AChE | ||||
| ATChI | 821 (782–860) | 146 (128–165) | 5.6 | n. d. |
| PTChI | 429 (415–442) | 150 (137–162) | 2.9 | n. d. |
n.d. = not determined, parentheses = 95% CI