Fig 4. Biolayer interferiometry shows a direct protein-protein interaction of NAF-1 and mNT with apo-Anamorsin.

Sensorgrams for the binding of holo-NAF-1 (A) and holo-mNT (B) to biotinylated apo-Anamorsin immobilized to streptavidin-coated biosensors are shown. The association was followed for 900 seconds (rising signal) followed by 1800 seconds of dissociation (decaying signal). The data was fit to a one-to-one model (black curves). On- and off-rates were determined from the fits for each NEET-Anamorsin concentration (shown in Table 2).