Table 1. Primers used for Real-Time PCR amplification of the genes studied in C. riparius.

Gene	Name	DNA sequence (5′-3′)	Fragment size (bp)	Efficiency (%)
actin	Forward	GATGAAGATCCTCACCGAACG	201	104
	Reverse	CGGAAACGTTCATTACCG
GAPDH	Forward	GGTATTTCATTGAATGATCACTTTG	110	96.6
	Reverse	TAATCCTTGGATTGCATGTACTTG
26S	Forward	TTCGCGACCTCAACTCATGT	220	90.4
	Reverse	CCGCATTCAAGCTGGACTTA
EcR	Forward	CCATCGTCATCTTCTCAG	180	106.6
	Reverse	TGCCCATTGTTCGTAG
usp	Forward	GCCCAATCATCCGTTAAGTGG	114	108.1
	Reverse	CGTTTGAAGAATCCTTTACATCC
E74	Forward	TCTTACTGAAACTTCTTCAAGATCG	111	103.2
	Reverse	GCTTTGAGACAGCTTTGGAATCG
vg	Forward	GATTGTTCCATGTGCAG	215	112.3
	Reverse	TTTGAGTATGGTGGAGAATC
hsp70	Forward	ACTTGAACCAGTTGAGCGT	132	103.8
	Reverse	TTGCCACAGAAGAAATCTTG
hsc70	Forward	CGTGCTATGACTAAGGACAA	239	99.3
	Reverse	GCTTCATTGACCATACGTTC
rpL13	Forward	AAGCTGCTTTCCCAAGAC	351	109.3
	Reverse	TTGGCATAATTGGTCCAG