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. 2015 Oct 8;10(10):e0139638. doi: 10.1371/journal.pone.0139638

Fig 2. Analysis of types of AngII receptors and G proteins involved in AngII regulation of LPL expression.

Fig 2

(A) VAT or SAT was pre-treated with 10 μM of candesartan (Cand), 5 μM of PD123177, or vehicle (Cont) for 1 h prior to AngII (1 μM) addition. (B) VAT or SAT was pre-treated with 100 nM YM25490 for 1 h or 100 ng/mL of PTX for 12 h prior to AngII (1 μM) addition. Control cells were treated with 12 h with PBS. After 24 h treatment with AngII, LPL mRNA expression was measured. Each column and bar represents the mean ± SEM for three separate experiments. An asterisk (*) indicates p<0.05 vs. without AngII. The LPL mRNA levels were normalized to β-actin.