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. 2015 Oct 8;10(10):e0139638. doi: 10.1371/journal.pone.0139638

Fig 6. PLCβ4 is highly expressed in VAT and visceral adipocytes.

Fig 6

Total protein (A, C) and total RNA (B, D) was extracted for western blot analysis of PLCβ4 protein and for qRT-PCR analysis of PLCβ4 mRNA, respectively (A and B, adipose tissue; C and D; adipocytes). Duplicated samples in each group were processed for western blotting. Each column and bar represents the mean ± SEM of 6 values from three separate experiments. An asterisk (*) indicates p<0.05 vs. VAT or visceral adipocytes. In panel A and C, the ratio of PLCβ4 to β-actin was calculated based on densitometric quantification of the bands. In panel B and D, PLCβ4 mRNA levels were normalized to β-actin.