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. 2015 Oct 8;10(10):e0139416. doi: 10.1371/journal.pone.0139416

Fig 4. Mitochondrial ROS dependent activation of mtDNA-TLR9 pathway in the heart after sepsis.

Fig 4

Rats were infected by S. pneumoniae or given PBS sham control. 21.5 μmoles/kg Mito-Vit-E (MVE) or vehicle was administered orally 30 minutes post-inoculation, and heart tissues were harvested 24 hours later. A. Levels of mtDNA in cytosol fractions were measured by real-time PCR. B. Expression of TLR9, IRAK4 and MyD88 were determined in total tissue lysates by western blot using GAPDH as a loading control, and the results were quantified by densitometry. All values are means ±SE. Significant differences are shown as * between sham and sepsis and Δ between vehicle and Mito-Vit-E (p<0.02, n = 6).