Fig 4. P2Y12R blockade impairs hepatic granulomatous eosinophilia.

Representative granuloma images from infected mice (Ai) untreated or (Aii) treated with clopidogrel. Slide sections were stained with Syrius red (modified method) and examined under a light microscope. (B) The number of eosinophils per granuloma area were calculated from images of 10–20 granulomas acquired for each individual mouse. Data represent the mean ± SE and were analyzed by Student’s t test. (*p<0.05). n = 10 mice/group and represents animals from at least 2 independent infection studies. (C) Immunofluorescence showing Siglec-F and P2Y12R staining of cytospin smears of murine eosinophil-enriched suspensions. P2Y12R, green; Siglec-F, red; Ab = antibody. Data are from one experiment, representative of 3 experiments. (D) Flow cytometry dot plots showing isotype control (left panel) and Siglec-F versus P2Y12R staining (right panel) of mice bone marrow derived eosinophils. Data are from one experiment, representative of 3 experiments.